BMS6012   3D Tissue Culture and Genome Editing   (15 credits)

Description

Many of the major pharmaceutical companies are using 3D models of disease and most use genome editing. This practical module will instruct students how to grow cells including 3D tumour spheroids in culture using different growth conditions. Students will learn how to transfect plasmid constructs to induce gene expression changes and use plasmids as molecular reporters of pathway activity. They will experience how to treat cells with RNAi and small molecules and measure the effect of gene expression. Students will grow 3D tissues and 3D tumours in this practical module and perform targeted gene studies. The students will use FACS for cell type selection and use CRISPR genome editing and knock-out techniques to build models of disease. At the end of this module students will have an extensive knowledge of 3D cell culture and the genome editing tools used in drug discovery and how to use them in screening campaigns.

Reading List